Green Fluorescent Protein: Properties, Applications and Protocols

Voorkant
Martin Chalfie, Steven R. Kain
John Wiley & Sons, 18 nov. 2005 - 450 pagina's
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications.

This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
 

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Inhoudsopgave

1 DISCOVERY OF GREEN FLUORESCENT PROTEIN
1
2 PHOTONS FOR REPORTING MOLECULAR EVENTS GREEN FLUORESCENT PROTEIN AND FOUR LUCIFERASE SYSTEMS
15
3 BIOCHEMICAL AND PHYSICAL PROPERTIES OF GREEN FLUORESCENT PROTEIN
39
4 THE THREEDIMENSIONAL STRUCTURE OF GREEN FLUORESCENT PROTEIN AND ITS IMPLICATIONS FOR FUNCTION AND DESIGN
67
5 MOLECULAR BIOLOGY AND MUTATION OF GREEN FLUORESCENT PROTEIN
83
6 DISCOVERY AND PROPERTIES OF GFPLIKE PROTEINS FROM NONBIOLUMINESCENT ANTHOZOA
121
7 EVOLUTION OF FUNCTION AND COLOR IN GFPLIKE PROTEINS
139
8 THE USES OF GREEN FLUORESCENT PROTEIN IN PROKARYOTES
163
11 GREEN FLUORESCENT PROTEIN APPLICATIONS IN DROSOPHILA
227
12 THE USES OF GREEN FLUORESCENT PROTEIN IN PLANTS
259
13 USES OF GFP IN TRANSGENIC VERTEBRATES
285
14 THE USES OF GREEN FLUORESCENT PROTEIN IN MAMMALIAN CELLS
305
15 PRACTICAL CONSIDERATIONS FOR USE OF REEF CORAL FLUORESCENT PROTEINS IN MAMMALIAN CELLS APPLICATIONS IN FL...
339
16 PHARMACEUTICAL APPLICATIONS OF GFP AND RCFP
361
17 REASSEMBLED GFP DETECTING PROTEINPROTEIN INTERACTIONS AND PROTEIN EXPRESSION PATTERNS
391
Methods and Protocols
407

9 THE USES OF GREEN FLUORESCENT PROTEIN IN YEASTS
179
10 USES OF GFP IN CAENORHABDITIS ELEGANS
203

Overige edities - Alles weergeven

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Over de auteur (2005)

MARTIN CHALFIE, PhD, is William R. Kenan Jr. Professor of Biological Sciences at Columbia University. He is world renowned for introducing the use of GFP as a biological marker. A member of the National Academy of Sciences, Dr. Chalfie serves on the editorial boards of Genome Biology and Molecular Biology of the Cell.

STEVEN R. KAIN, PhD, manages the Applications Gene Expression Group for Agilent Technologies. While director of cell biology at Clontech Laboratories, he was responsible for commercializing the first products based on GFP and produced the popular EGFP variant. He has twelve years' experience with a variety of biotechnology firms in the San Francisco Bay Area.

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