Molecular Microbiology Laboratory: A Writing-Intensive CourseAcademic Press, 31 aug 2012 - 259 pagina's Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.
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Inhoudsopgave
Introduction | xi |
Experiment 1 Plasmid Purification and Restriction Mapping | 1 |
Experiment 2 Affinity Purification of HistidineTagged FnbA Protein | 29 |
Experiment 3 Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes | 43 |
Experiment 4 Terminal Restriction Fragment Length Polymorphism Analysis of 16S Ribosomal RNA Genes from Uncultured Bacteria | 71 |
Appendices | 103 |
| 253 | |
| 255 | |
Overige edities - Alles bekijken
Molecular Microbiology Laboratory: A Writing-Intensive Course Bruce Geller,Katherine Field Gedeeltelijke weergave - 2012 |
Veelvoorkomende woorden en zinsdelen
1.5-ml microcentrifuge tubes 16S rRNA genes 72 students acetate agar plates agarose gel electrophoresis ampicillin ampicillin-resistant amplicons autoclave bacterial populations band BLAST search cells coli colonies concentration culture delbrueckii diluted distilled water DNA mass ladder DNA molecules DNA sequence dNTP EcoRI EDTA endonuclease ethanol ethidium bromide experiment FIGURE FnbA freezer His-tagged Incubate indicate inoculate Invitrogen isolated Items per student labeled laboratory report Lac2 LacZ lane large gels luciferase lux operon mg/ml microcentrifuge tubes mini-gel minutes MspI no-template control Notes Notes nucleotides P20 pipetman PCR amplicons PCR primers PCR reaction pellet pipette pKN800 plasmid DNA plan for 72 probiotic Professor’s protein PstI PstI-cut purified PCR product Qiagen reagents restriction fragments room temperature sample seat number Select sentence sterile distilled water supernatant solution T-RFLP analysis T-RFLP fragment T-RFLP peaks TA’s role thermal cycler thermophilus transformants uncut pKN800 plasmid unit vector yellow tips yogurt Yoplaits