Gene BiotechnologyCRC Press, 29 sep 2003 - 536 pagina's Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development. C |
Inhoudsopgave
| 15 | |
Chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries | 31 |
Chapter 4 Subcloning of Genes or DNA Fragments | 73 |
Chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencing | 99 |
Chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteins | 139 |
Chapter 7 Characterization of DNA or Genes by Southern Blot Hybridization | 165 |
Chapter 8 Gene Overexpression by Sense RNA in Mammalian Systems | 193 |
Chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies | 223 |
Chapter 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RTPCR Detection of Low Abundance mRNAs | 323 |
Chapter 15 Isolation and Characterization of Genes from Genomic DNA Libraries | 341 |
Chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression | 365 |
Chapter 17 New Strategies for Gene Knockout | 375 |
Chapter 18 LargeScale Expression and Purification of Recombinant Proteins in Cultured Cells | 409 |
Chapter 19 Quantitative Analysis of Functional Genome by Current RealTime RTPCR | 445 |
Chapter 20 HighThroughput Analysis of Gene Expression by Cutting Edge Technology 8212 DNA Microarrays Gene Chips | 461 |
Using StateoftheArt Technology 8212 Phage Display | 471 |
Chapter 10 Analysis of Gene Expression at the Functional Genomic Level | 247 |
Chapter 11 Analysis of Gene Expression at the Proteomic Level | 265 |
Chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridation RISH | 287 |
Chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization FISH | 311 |
Overige edities - Alles bekijken
Gene Biotechnology, Second Edition William Wu,Helen H. Zhang,Michael J. Welsh,Peter B. Kaufman Geen voorbeeld beschikbaar - 2003 |
Veelvoorkomende woorden en zinsdelen
acid activity agarose allow amplification analysis antibodies antisense appropriate approximately bacteriophage bands blot bottom buffer Carefully carried cDNA cells Centrifuge Chapter cloning complete concentration constructs containing culture denatured described designed detailed detection digestion dilution dish Dissolve EDTA electrophoresis ethanol expression extract Figure filter final fragments fresh gene glass hybridization Incubate insert interest isolation labeled lane ligation loading medium membrane method mg/ml mixture mRNA NaCl Needed Note Once partially pipette plasmid plate polymerase positive precipitate Preparation Press primer prior probe procedures promoter protein Protocol reaction Reagents recombinant region remove restriction enzyme result reverse RNase room temperature sample screening selection sequence signal slides solution specific step sterile Store strand supernatant Take template Tips tissue transfected transfer transformation Tris-HCl tube vector volume washing western blot