Gene Transfer and Expression in Mammalian Cells
The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.
This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.
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Product recovery and initial purification
Chromatographic purification of recombinant proteins
Product stability and longterm storage
Overige edities - Alles weergeven
AAV vectors Acad acid activity antibody antigen apoptosis B-globin baculovirus binding Biol Biotechnol capsid caspases cassette cDNA cell lines cellular Chem CHO cells chromatin chromatin insulators chromosomal clones codon complex containing copy number cytoplasm degradation domain effects efficiency elements encoding enhancer enzymes expression in mammalian expression levels expression vectors factors function fusion gene delivery gene expression Gene Ther gene therapy gene transfer genetic genome glycans glycosylation heterologous host cell human immune response infection inhibition integration interaction intrabody lipid mammalian cells membrane mice molecules mRNA mutations Natl nuclear packaging particles pathway plasmid poliovirus Proc promoter proteasome receptor recombinant proteins region regulation regulatory replication replicon reporter gene retroviral ribosome selection sequences SFV vectors signal specific stability structure substrate target transcription transduction transfection transgene transgene expression translation ubiquitin vaccine viral genome virion Virol virus viruses vitro vivo