Methods in Extracellular Matrix Biology

Voorkant
Academic Press, 5 jan 2018 - 468 pagina's

Methods in Extra Cellular Matrix, Volume 142, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections devoted to Elastin, Quantification of collagen and elastin, Fibrillins, Lysyl oxidase, Fibulins, Matrilins, Hyaluronic Acid, Small leucine-rich proteoglycans, Syndecans, Fibronectin, SPARC, Thrombospondins, Tenascins, Collagen IV, Multi-photon analysis of ECM, Cell-derived extracellular matrices, Laminins, Fibrillar Collagens, Imaging ECM in developing embryos, Analysis of Matrix Degradation, Ultrastructural analysis of ECM, Versican and Large proteoglycans, and an ECM crosslink analysis.

This series covers a wide array of topics about the extracellular matrix, including an understanding of crucial proteins and glycoproteins components of ECM.

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on the extracellular matrix, including an understanding crucial proteins and the glycoproteins components of ECM
  • Includes analysis based topics, such as quantification of collagen and elastin, mulit-photon analysis of ECM and ECM crosslink analysis
 

Inhoudsopgave

Ultrastructural analysis of the extracellular matrix
1
Live tissue antibody injection A novel method for imaging ECM in limb buds and other tissues
41
Nonlinear optical imaging of extracellular matrix proteins
57
Methods for the visualization and analysis of extracellular matrix protein structure and degradation
79
Cellderived decellularized extracellular matrices
97
Analysis of collagen and elastin crosslinks
115
Measurement of elastin collagen and total protein levels in tissues
133
Measurement of lysyl oxidase activity from small tissue samples and cell cultures
147
Expression and purification of recombinant fibulins in mammalian cells
247
The biochemistry and immunohistochemistry of versican
261
Isolation production and analysis of small leucinerich proteoglycans in bone
281
Quantification of hyaluronan HA using a simplified fluorophoreassisted carbohydrate electrophoresis FACE pr
297
Isolation and functional analysis of syndecans
317
Production and purification of recombinant human SPARC
335
Thrombospondins Purification of human platelet thrombospondin1
347
How to detect and purify tenascinC
371

Microtiter assays for quantitation of assembly of plasma and cellular fibronectin
157
Basement membrane collagen IV Isolation of functional domains
171
Isolation and analysis of laminins
187
Elastin purification and solubilization
207
Fibrillins
223
Investigating celltype specific functions of tenascinC
401
Matrilins
429
Back Cover
447
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Over de auteur (2018)

Dr. Robert Mecham is the Alumni Endowed Professor of Cell Biology and Physiology at Washington University School of Medicine. He holds joint appointments at the professorial level in Medicine, Pediatrics, and Bioengineering. He received his Bachelor of Science degree in Biology at the University of Utah in 1973 where he developed an interest in the extracellular matrix proteins that contribute to vascular function. He continued these studies during his graduate work at Boston University School of Medicine where he earned a Ph.D. in Biochemistry in 1977. Dr. Mecham was recruited to Washington University in 1977 in the Pulmonary Division, Department of Medicine, where his research focused on the underlying causes of emphysema and pulmonary hypertension. After a sabbatical at the National Institutes of Health as a Visiting Scientist, Dr. Mecham moved to the Department of Cell Biology and Physiology at Washington University where his laboratory continues to study extracellular matrix structure and assembly. Dr. Mecham has authored over 250 scientific papers and has edited 13 books. He was the first president of the American Society for Matrix Biology and has two honorary degrees from universities in Europe. He is a fellow of the American Association for the Advancement of Science and has served on editorial boards of major scientific journals. He has held numerous national and international scientific leadership positions and has served on important review and policy committees at all levels. At Washington University, Dr. Mecham twice served as interim chair of his Department. He has extensive involvement in medical and graduate school education and has received the university’s Distinguished Teacher Award three times and the Outstanding Faculty Mentor Award. Over 40 students and fellows have trained in his laboratory and have gone on to research and teaching positions in universities throughout the world. In recognition of his outstanding research and contributions to Washington University, Dr. Mecham was recently awarded the Carl and Gerty Cori Faculty Achievement Award.

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