Methods in Extracellular Matrix BiologyAcademic Press, 5 jan 2018 - 468 pagina's Methods in Extra Cellular Matrix, Volume 142, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections devoted to Elastin, Quantification of collagen and elastin, Fibrillins, Lysyl oxidase, Fibulins, Matrilins, Hyaluronic Acid, Small leucine-rich proteoglycans, Syndecans, Fibronectin, SPARC, Thrombospondins, Tenascins, Collagen IV, Multi-photon analysis of ECM, Cell-derived extracellular matrices, Laminins, Fibrillar Collagens, Imaging ECM in developing embryos, Analysis of Matrix Degradation, Ultrastructural analysis of ECM, Versican and Large proteoglycans, and an ECM crosslink analysis. This series covers a wide array of topics about the extracellular matrix, including an understanding of crucial proteins and glycoproteins components of ECM.
|
Inhoudsopgave
1 | |
41 | |
Nonlinear optical imaging of extracellular matrix proteins | 57 |
Methods for the visualization and analysis of extracellular matrix protein structure and degradation | 79 |
Cellderived decellularized extracellular matrices | 97 |
Analysis of collagen and elastin crosslinks | 115 |
Measurement of elastin collagen and total protein levels in tissues | 133 |
Measurement of lysyl oxidase activity from small tissue samples and cell cultures | 147 |
Expression and purification of recombinant fibulins in mammalian cells | 247 |
The biochemistry and immunohistochemistry of versican | 261 |
Isolation production and analysis of small leucinerich proteoglycans in bone | 281 |
Quantification of hyaluronan HA using a simplified fluorophoreassisted carbohydrate electrophoresis FACE pr | 297 |
Isolation and functional analysis of syndecans | 317 |
Production and purification of recombinant human SPARC | 335 |
Thrombospondins Purification of human platelet thrombospondin1 | 347 |
How to detect and purify tenascinC | 371 |
Microtiter assays for quantitation of assembly of plasma and cellular fibronectin | 157 |
Basement membrane collagen IV Isolation of functional domains | 171 |
Isolation and analysis of laminins | 187 |
Elastin purification and solubilization | 207 |
Fibrillins | 223 |
Investigating celltype specific functions of tenascinC | 401 |
Matrilins | 429 |
Back Cover | 447 |