Green Fluorescent Protein: Properties, Applications, and Protocols
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications.
This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
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BIOCHEMICAL AND PHYSICAL PROPERTIES OF GREEN
THE THREEDIMENSIONAL STRUCTURE OF GREEN FLUORESCENT
MOLECULAR BIOLOGY AND MUTATION OF GREEN
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Green Fluorescent Protein: Properties, Applications and Protocols
Martin Chalfie,Steven R. Kain
Gedeeltelijke weergave - 2005
Acad activity Aequorea GFP Aequorea victoria aequorin amino acid analysis Anthozoa Arabidopsis assays bacteria Biochem Biochemistry bioluminescence Biotechnol Caenorhabditis elegans Cell Biol cellular Chalfie chromophore chromosome cloned Cnidaria color confocal coral Cormier detection dimer domains Drosophila DsRed dynamics elegans embryos emission energy transfer excitation expressing GFP fluorophore FRET function fused fusion protein gene expression genetic GFP expression GFP fluorescence GFP fusion proteins GFP variants GFP-like proteins green fluorescent protein HcRedl Heim imaging labeled living cells localization luciferase luciferin Lukyanov luminescence mammalian cells marker Matz membrane microscopy molecular molecules Morin mutants Natl neurons nuclear peak peptide photobleaching plasmid Prasher Proc promoter protein-protein interactions quantum yield RCFPs receptor recombinant red fluorescent protein residues screening sequence Shimomura signal specific spectral subcellular target tion tissue transcription transfected transgenic Tsien vectors visualized vivo Ward wavelengths wild-type GFP yeast zebrafish