Methods in Cellular ImagingAmmasi Periasamy Springer, 27 mei 2013 - 448 pagina's Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided. |
Inhoudsopgave
Fluorophores and Their Labeling Procedures for Monitoring Various | 20 |
Detectors for Fluorescence Microscopy | 40 |
Basics of a Light Microscopy Imaging System and Its Application | 53 |
Laser Scanning Confocal Microscopy Applied to Living Cells | 66 |
Cell and Molecular Imaging | 84 |
Functional Imaging of Mitochondria Within Cells | 88 |
Processing MicroscopeAcquired Images for Use in Multimedia Print | 128 |
Introduction | 145 |
Cellular Response to Laser Radiation in Fluorescence Microscopes | 236 |
Fluorescence Resonance Energy Transfer and Lifetime | 252 |
Carnegie Mellon University Victoria Australia | 257 |
WideField Confocal TwoPhoton and Lifetime Resonance Energy | 295 |
One and TwoPhoton Confocal Fluorescence Lifetime Imaging | 309 |
Biological Applications of TimeResolved PumpProbe Fluorescence | 324 |
Introduction | 343 |
Total Internal Reflection Fluorescence Microscopy | 362 |
Building a TwoPhoton Microscope Using a Laser Scanning Confocal | 162 |
ALBERTO DIASPRO Ph D TODD FRENCH Ph D | 168 |
TwoPhoton Microscopy in Highly Scattering Tissue | 180 |
Nuclear Magnetic Resonance Center ENRICO GRATTON Ph D | 186 |
Multiphoton Laser Scanning Microscopy and Dynamic Imaging | 200 |
In vivo Diffusion Measurements Using Multiphoton Excitation | 216 |
Overige edities - Alles bekijken
Methods in Cellular Imaging Ammasi Periasamy,American Physiological Society (1887- ) Geen voorbeeld beschikbaar - 2001 |
Veelvoorkomende woorden en zinsdelen
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