Single Molecule Tools, Part A: Fluorescence Based ApproachesAcademic Press, 17 aug 2010 - 528 pagina's Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.
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AzideSpecific Labeling of Biomolecules by StaudingerBertozzi Ligation Phosphine Derivatives of Fluorescent Probes Suitable for SingleMolecule Fl... | 19 |
Preparation of Fluorescent PremRNA Substrates for an smFRET Study of PremRNA Splicing in Yeast | 31 |
Nanovesicle Trapping for Studying Weak Protein Interactions by SingleMolecule FRET | 41 |
Droplet Confinement and Fluorescence Measurement of Single Molecules | 61 |
A Highly Purified Fluorescently Labeled In Vitro Translation System for SingleMolecule Studies of Protein Synthesis | 221 |
Watching Individual Proteins Acting on Single Molecules of DNA | 261 |
DNA Curtains for HighThroughput SingleMolecule Optical Imaging | 293 |
Scanning FCS for the Characterization of Protein Dynamics in Live Cells | 317 |
Observing Protein Interactions and Their Stoichiometry in Living Cells by Brightness Analysis of Fluorescence Fluctuation Experiments | 345 |
Detection of Individual Endogenous RNA Transcripts In Situ Using Multiple Singly Labeled Probes | 365 |
Single mRNA Tracking in Live Cells | 387 |
SingleMolecule Sequencing Sequence Methods to Enable Accurate Quantitation | 407 |
SingleMolecule Fluorescence Spectroscopy Using Phospholipid Bilayer Nanodiscs | 89 |
SingleMolecule Spectroscopy Using Microfluidic Platforms | 119 |
Detection of ProteinProtein Interactions in the Live Cell Plasma Membrane by Quantifying Prey Redistribution upon Bait Micropatterning | 133 |
Analysis of Complex SingleMolecule FRET Time Trajectories | 153 |
SingleMolecule Fluorescence Studies of Intrinsically Disordered Proteins | 179 |
Measuring the Energetic Coupling of Tertiary Contacts in RNA Folding using Single Molecule Fluorescence Resonance | 205 |
RealTime DNA Sequencing from Single Polymerase Molecules | 431 |
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Color Plate | 477 |
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